Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

HiTrap^® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose^® High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Features and Benefits

Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series. Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose^® High Performance. Recovery and purity of IgY are excellent. Can be operated with a liquid chromatography system such as ÄKTA^® design. The top and bottom frits are manufactured from porous polyethylene Connectors for usage with different chromatography systems and other equipment are included

General description

HiTrap^® IgY Purification HP is a 5 mL column prepacked with Sepharose^® High Performance for fast and easy purification of IgY from egg yolk.

Legal Information

Sepharose is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

HiTrap is a registered trademark of Cytiva